Background:

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Specificity:

NM23 monoclonal antibody detects endogenous levels of NM23 protein.

Alternative names:

NDK A, NDP kinase A, GAAD, NME1, NDPKA, NM23, AWD, AWD, drosophila, homolog of, GAAD, Granzyme A activated DNase, Granzyme A-activated DNase, GZMA activated DNase, Metastasis inhibition factor NM23, NB, NBS, NDK A, NDKA, NDKA, NDP kinase A, NDPK-A, NDPKA, NM23, NM23 long variant, included, nm23-H1, NM23-M1, NM23H1B, included, NME/NM23 nucleoside diphosphate kinase 1, Nme1, NME1-NME2 spliced read-through transcript, included, Non-metastatic cells 1, protein (NM23A) expressed in, Nonmetastatic cells 1, protein expressed in, Nonmetastatic protein 23, Nonmetastatic protein 23, homolog 1, Nucleoside diphosphate kinase A, Tumor metastatic process-associated protein antibody

Species reactivity:

Human

Host:

Mouse

Ig type:

Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide within human NM23 aa 80-120.

Purification & Purity:

Protein G purified.

Molecular weight:

17/19 kDa

Applications:

WB ICC IHC

Usage:

WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200

Storage:

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com