Background:

Betaine-homocysteine methyltransferase (BHMT) is a zinc-dependent cytosolic protein that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT is highly expressed in rat liver, and its expression is regulated by dietary methionine and choline. In humans, S-adenosylmethionine (SAM) down-regulates BHMT expression by inducing NFκB, which acts as a repressor for the BHMT gene. Lowered BHMT levels can lead to ER (endoplasmic reticulum) stress. Mutations in the gene encoding for BHMT may lead to hyperhomocysteinemia, a medical condition characterized by abnormally large amounts of homocysteine in the blood which may be a risk factor for cardiovascular and cerebrovascular diseases.

Specificity:

BHMT monoclonal antibody detects endogenous levels of BHMT protein.

Alternative names:

BHMT, Betaine homocysteine methyltransferase, Betaine homocysteine S methyltransferase 1, Betaine homocysteine S methyltransferase, Betainehomocysteine S-methyltransferase 1, BHMT, BHMT1, BHMT1, EC 2.1.1.5, Epididymis secretory sperm binding protein Li 61p, HEL S 61p,

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Ig type:

Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein.

Purification & Purity:

ProG affinity purified.

Molecular weight:

45 kDa

Applications:

Usage:

WB:1:1,000-1:5,000 ICC:1:50 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com