Background:

The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax p21 (for Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Specificity:

This antibody detects endogenous levels of Bax and does not cross-react with related proteins.

Alternative names:

Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BAX; BCL2L4

Species reactivity:

Human,mouse

Host:

Mouse

Ig type:

Mouse IgA, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to N-terminal Bax.

Purification & Purity:

Protein A affinity purified

Molecular weight:

~ 21 kDa

Applications:

WB ICC IHC FC

Usage:

WB: 1:500 ICC: 1:200 IHC: 1:200 FC: 1:50

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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