Background:

The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication, and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli, however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms, one involved in ongoing DNA replication. which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.

Specificity:

This antibody detects endogenous levels of PCNA and does not cross-react with related proteins.

Alternative names:

Proliferating cell nuclear antigen; Cyclin

Species reactivity:

Human,Mouse,Rat

Host:

Mouse

Ig type:

Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

This antibody is produced by immunizing mice with recombinant protein corresponding to a region of PCNA.

Purification & Purity:

Protein A affinity purified

Molecular weight:

~ 29, 36 kDa

Applications:

WB IHC FC

Usage:

WB: 1:1000-1:2000 IHC: 1:200 FC:1:100

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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