Background:

Epitope tagging is a widely accepted technique that involves the fusion of an epitope labeled peptide to a certain protein as a marker for gene expression. This technique allows gene expression to be easily monitored during western blotting, immunoprecipitation and immunofluorescence by using an antibody that recognizes such an epitope. Amino acid sequences that are widely used for epitope tagging are as follows; YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VSV-G-Tag), which correspond to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product, and Vesicular stomatitis virus glycoprotein, respectively.

Specificity:

VSV-G-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal VSV-G- tagged proteins.

Alternative names:

VSVG

Species reactivity:

All

Host:

Mouse

Ig type:

Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide VSV-G-Tag (YTDIEMNRLGK).

Purification & Purity:

Affinity-chromatography

Molecular weight:

N/A

Applications:

WB IF

Usage:

WB: 1:5000~1:10000 IF: 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com