Background:

The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors that is based on the very efficient T7 RNA polymerase expression system. Antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.

Specificity:

T7-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal T7- tagged proteins.

Alternative names:

T7 Tag; MASMTGGQQMG epitope tag; Enterobacteria phage T7; Bacteriophage T7; Minor capsid protein 10B; Major capsid protein 10A

Species reactivity:

All

Host:

Mouse

Ig type:

Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide T7-Tag.

Purification & Purity:

Affinity-chromatography

Molecular weight:

N/A

Applications:

WB IF

Usage:

WB: 1:5000~1:10000 IF: 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com