Background:

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.

Specificity:

Strep Ⅱtag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal Strep Ⅱ- tagged proteins.

Alternative names:

Strep-Tag II; Strep II

Species reactivity:

All

Host:

Mouse

Ig type:

Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide Strep II Tag.

Purification & Purity:

Affinity-chromatography

Molecular weight:

N/A

Applications:

WB IF

Usage:

WB: 1:5000~1:10000 IF: 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com