Background:

AMPKβ1 is a member of the SNF1 kinase family. It is a heterotrimeric protein comprising of an alpha (catalytic), beta (non-catalytic) and gamma (non-catalytic) subunits, 63, 38 and 38kDa respectively.AMPKβ1 regulates fatty acid and sterol synthesis by phosphorylation of acteyl-coA. It also regulates cholesterol synthesis via phosphorylation and in-activation of hydroxymethylglutaryl-CoA reductase.Two isoforms of AMPKβ1 have been found.

Specificity:

This antibody detects endogenous levels of AMPKβ1 and does not cross-react with related proteins.

Alternative names:

5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb; PRKAB1; AMPK; AMPK β1

Species reactivity:

Human,Mouse,Rat,Monkey

Host:

Mouse

Ig type:

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Purified recombinant human AMPKβ1 protein fragments expressed in E.coli.

Purification & Purity:

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

Predicted band size:38KDa Observed band size:38KDa

Applications:

WB ICC IHC IP

Usage:

WB: 1:1000 IP: 1:50~200 ICC: 1:200 IHC: 1:800

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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Email: info@sobekbio.com

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