Background:

The RAD23 genes (RAD23A and RAD23B), which encode the human Rad23 proteins, are crucial for excision-repair of UV-damaged DNA. RAD23 genes resemble the other DNA repair genes, RAD2, RAD6, RAD7, RAD18 and RAD54, all of which also exhibit increased transcription in response to DNA damage and during meiosis. Rad23 is a nuclear protein containing an ubiquitin-like domain required for biological functions. The protein, which is highly conserved, is involved in nucleotide excision repair (NER) that associates with the proteasome via its N-terminus. The C-terminal ubiquitin-associated domain of Rad23 is evolutionarily conserved from yeast to humans. Rad23 may also act as a regulator for the activity of the 26S Proteasome.

Specificity:

This antibody detects endogenous levels of Rad23B and does not cross-react with related proteins.

Alternative names:

UV excision repair protein RAD23 homolog B; HR23B; hHR23B; XP-C repair-complementing complex 58 kDa protein; p58; RAD23B

Species reactivity:

Human,Mouse,Rat,Monkey

Host:

Mouse

Ig type:

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Purified recombinant human Rad23B protein fragments expressed in E.coli.

Purification & Purity:

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

Predicted band size:55KDa Observed band size:55KDa

Applications:

WB ICC IHC

Usage:

WB: 1:1000 IHC/ICC: 1:50~200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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