Background:

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.

Specificity:

This antibody detects endogenous levels of CHD3 (C-terminus) and does not cross-react with related proteins

Alternative names:

Chromodomain-helicase-DNA-binding protein 3; CHD-3; Hzfh; Zinc finger helicase; Mi2-alpha; ATP-dependent helicase CHD3

Species reactivity:

Human

Host:

Mouse IgG1

Ig type:

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to the C-terminus of Human CHD3.

Purification & Purity:

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

Predicted band size:226KDa Observed band size:260KDa

Applications:

WB IHC FC

Usage:

WB: 1:1000 IHC: 1:500 FC: 1:50~100

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com