Background:

Protein phosphatase 4C may be involved in microtubule organization. It binds 1 iron ion and 1 manganese ion per subunit. PP4 consists of a catalytic subunit PPP4C and a regulatory subunit. PPP4R1 and belongs to the PPP phosphatase family, PP X subfamily.Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity)

Specificity:

This antibody detects endogenous levels of PPP4C and does not cross-react with related proteins

Alternative names:

Serine/threonine-protein phosphatase 4 catalytic subunit; PP4C; Pp4; Protein phosphatase X; PP-X; PPP4C; PPP4; PPX

Species reactivity:

Human

Host:

Mouse

Ig type:

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human PPP4C.

Purification & Purity:

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

Predicted band size:34KDa Observed band size:34KDa

Applications:

WB IHC ICC

Usage:

WB: 1:1000 ICC: 1:50~200 IHC: 1:500

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com