Background:

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer.

Specificity:

TWIST1 monoclonal antibody detects endogenous levels of TWIST1 protein.

Alternative names:

Twist-related protein 1; Class A basic helix-loop-helix protein 38; bHLHa38; H-twist; BHLHA38; TWIST

Species reactivity:

Human,Mouse

Host:

Mouse

Ig type:

Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Purified recombinant fragment of human TWIST1 (AA: 9-74) expressed in E. Coli.

Purification & Purity:

Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer

Molecular weight:

~ 22 kDa

Applications:

WB IHC ICC FC

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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