Background:

Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule.

Specificity:

This antibody detects ubiquitin, polyubiquitin and ubiquitinated proteins.

Alternative names:

Polyubiquitin-B; Ubiquitin; UBB; Polyubiquitin-C; UBC; RPS27A; UBA80; UBCEP1; CEP52; UBA52; UBCEP2

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant antibody.

Purification & Purity:

Protein A affinity purified

Molecular weight:

~ *8 kDa or Uncertain

Applications:

WB ICC/IF IHC FC

Usage:

WB: 1:1000-1:2000 ICC: 1:50-1:200 IHC: 1:200-1:500 FC: 1:50-1:100

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com