Background:

CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. CD34 antigen expression is highest in the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation consensus sites.

Specificity:

This antibody detects endogenous levels of CD34 and does not cross-react with related proteins.

Alternative names:

CD34; Cd34; CD34 antigen; CD34 molecule; CD34; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; Mucosialin;

Species reactivity:

Human,Mouse,Rat, Dog

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant antibody.

Purification & Purity:

Protein A affinity purified

Molecular weight:

~ 41, 120 kDa

Applications:

WB ICC/IF IHC

Usage:

WB: 1:1000-1:2000 ICC/IF: 1:50-1:200 IHC: 1:50-1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com