Background:

H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.

Specificity:

This antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139.

Alternative names:

Histone H2AX; H2a/x; Histone H2A.X; H2AFX; H2AX; γ-H2AX; γH2AX; ganunaH2AX; γ-H2A.X; γH2A.X; ganunaH2A.X

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant antibody.

Purification & Purity:

Protein A affinity purified

Molecular weight:

~ 15 kDa

Applications:

WB IHC

Usage:

WB: 1:1000-1:5000 IHC: 1:50-1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com