Background:

Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.

Specificity:

This antibody detects endogenous levels of NeuN and does not cross-react with related proteins.

Alternative names:

RNA binding protein fox-1 homolog 3; Fox-1 homolog C; RBFOX3; NeuN; Fox-3; Fox3; Neuronal Marker

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant antibody.

Purification & Purity:

Protein A affinity purified

Molecular weight:

Predicted band size:34KDa Observed band size:46, 48KDa

Applications:

WB ICC/IF IHC FC

Usage:

WB: 1:1000-1:2000 ICC/IF: 1:50-1:200 IHC: 1:200-1:500 FC: 1:50-1:100

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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Email: info@sobekbio.com

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