Background:
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Specificity:
This antibody detects endogenous levels of Histone H1.3 or H1.4 protein only when pho17.
Alternative names:
Histone H1.4; Histone H1b; Histone H1s-4; HIST1H1E; H1F4; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1D; H1F3
Species reactivity:
Human,Mouse,Rat
Host:
Rabbit
Ig type:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Immunogen:
Recombinant antibody.
Purification & Purity:
Protein A affinity purified
Molecular weight:
~ 30 kDa
Applications:
WB ICC/IF IHC
Usage:
WB: 1:500-1:1000
IHC/ICC/IF: 1:50-1:200
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Precautions:
For research use only, not for use in diagnostic procedure.
More info:
Email: info@sobekbio.com
Orders:
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