Background:

β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.

Specificity:

This antibody detects endogenous levels of Catenin-β and does not cross-react with related proteins.

Alternative names:

Catenin β; Cateninβ; Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; ORF Names: OK/SW-cl.35; PRO2286

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant antibody.

Purification & Purity:

Protein A affinity purified

Molecular weight:

~ 92 kDa

Applications:

WB ICC IHC IP

Usage:

WB: 1:500-1:5000 ICC: 1:20-1:50 IHC: 1:20-1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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