Background:

Estrogen receptor alpha (ERa, ER, ESR, ESRA, Era, NR3A1, estrogen receptor 1) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding and activation of transcription. Alternative splicing results in several ERa mRNA transcripts, which differ primarily in their 5' untranslated regions. ERa undergoes phosphorylation in response to estradiol binding. Human ERa is predominately phosphorylated on Ser 118 and to a lesser extent on Ser 104 and Ser 106. In response to activation of the mitogen-activated protein kinase pathway, phosphorylation occurs on Ser 118 and Ser 167. These serine residues are all located within the activation function 1 region of the N-terminal domain of ERa. In contrast, activation of protein kinase A increases the phosphorylation of Ser 236, which is located in the DNA-binding domain. Src kinase-dependent Tyr 537 phosphorylation may enhance estrogen binding to ERa. Mutation of Tyr 537 of the human ERa produces receptors having a range of constitutive activity.

Specificity:

ERα (Phospho-S118) polyclonal antibody detects endogenous levels of ERα protein only when phosphorylated at S118.

Alternative names:

ER, ESR1, DKFZp686N23123, ER alpha, ER, ER-alpha, Era, ESR, ESR1, ESR1, ESRA, Estradiol receptor, Estrogen nuclear receptor alpha, Estrogen receptor 1, Estrogen receptor alpha 3*, 4, 5, 6, 7*/822 isoform, Estrogen receptor alpha, Estrogen receptor alpha delta 3*, 4, 5, 6, 7*, 8*/941 isoform, Estrogen receptor alpha delta 3*, 4, 5, 6, 7*/819 2 isoform, Estrogen receptor alpha delta 4 +49 isoform, Estrogen receptor alpha delta 4*, 5, 6, 7*/654 isoform, Estrogen receptor alpha delta 4*, 5, 6, 7, 8*/901 isoform, Estrogen receptor alpha E1 E2 1 2, Estrogen receptor alpha E1 N2 E2 1 2, Estrogen receptor, ESTRR, NR3A1, Nuclear receptor subfamily 3 group A member 1,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic phospho-peptide corresponding to residues surrounding Ser118 of human Estrogen Receptor alpha.

Purification & Purity:

ProA affinity purified

Molecular weight:

66 kDa

Applications:

WB ICC/IF IHC

Usage:

WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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