Background:

c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.

Specificity:

c-Myc (Phospho-T58/S62) polyclonal antibody detects endogenous levels of c-Myc protein only when phosphorylated at T58/S62.

Alternative names:

bHLHe39, MYC, Avian myelocytomatosis viral oncogene homolog, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, MRTL, Myc, Myc protein, Myc proto oncogene protein, Myc proto-oncogene protein, myc related translation/localization regulatory factor, MYC, Myc2, MYCC, Niard, Nird, Proto-oncogene c-Myc, Transcription factor p64, v myc avian myelocytomatosis viral oncogene homolog, v myc myelocytomatosis viral oncogene homolog,

Species reactivity:

Human, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human c-Myc.

Purification & Purity:

ProA affinity purified

Molecular weight:

49 kDa

Applications:

WB ICC/IF IP

Usage:

WB:1:1,000 ICC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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