Background:

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca²⁺. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

Specificity:

PKCα (Phospho-T638) polyclonal antibody detects endogenous levels of PKCα protein only when phosphorylated at T638.

Alternative names:

PKC-A, PKC-alpha, PRKCA, AAG6, Aging associated gene 6, aPKC, KPCA, PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, PRKCA, Protein Kinase C alpha, Protein kinase C alpha type,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha.

Purification & Purity:

ProA affinity purified

Molecular weight:

77 kDa

Applications:

WB ICC/IF IHC IP

Usage:

WB:1:1,000-1:5,000 ICC:1:50-1:500 IHC:1:50-1:500

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com