Background:

AMPK (5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. AMPKα1 (5'-AMP-activated protein kinase catalytic subunit alpha-1), also known as PRKAA1, is a 559 amino acid protein that belongs to the CAMK Ser/Thr protein kinase family and protein kinase superfamily. Highly phosphorylated, AMPKα1 exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 5p13.1.

Specificity:

AMPKα1 (Phospho-S496) polyclonal antibody detects endogenous levels of AMPKα1 protein only when phosphorylated at S496.

Alternative names:

AMPK subunit alpha-1, ACACA kinase, HMGCR kinase, PRKAA1, 5 AMP activated protein kinase alpha 1catalytic subunit, 5 AMP activated protein kinase catalytic alpha 1 chain, 5' AMP activated protein kinase catalytic subunit alpha 1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AAPK1, AAPK1, ACACA kinase, acetyl CoA carboxylase kinase, AI194361, AI450832, AL024255, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha -1, AMPK 1, AMPK alpha 1, AMPK alpha 1 chain, AMPK, AMPK subunit alpha-1, AMPK1, AMPKa1, AMPKalpha1, C130083N04Rik, cb116, EC 2.7.11.1, HMG CoA reductase kinase, HMGCR kinase, hormone sensitive lipase kinase, Hydroxymethylglutaryl CoA reductase kinase, im:7154392, kinase AMPK alpha1, MGC33776, MGC57364, OTTHUMP00000161795, OTTHUMP00000161796, PRKAA 1, PRKAA1, Protein kinase AMP activated alpha 1 catalytic subunit, SNF1-like protein AMPK, SNF1A, Tau protein kinase PRKAA1, wu:fa94c10,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic phospho-peptide corresponding to residues surrounding Ser496 of human AMPK alpha 1.

Purification & Purity:

ProA affinity purified

Molecular weight:

64 kDa

Applications:

WB ICC/IF IP

Usage:

WB:1:1,000-1:5,000 ICC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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