Background:

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.

Specificity:

MLKL (phospho-S345) polyclonal antibody detects endogenous levels of MLKL protein only when phosphorylated at S345.

Alternative names:

Mlkl, 9130019I15Rik, FLJ34389, hMLKL, Mixed lineage kinase domain like, Mixed lineage kinase domain like protein, Mixed lineage kinase domain like pseudokinase, Mixed lineage kinase domain-like protein, Mlkl, MLKL,

Species reactivity:

Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic phospho-peptide corresponding to residues surrounding Ser345 of human MLKL.

Purification & Purity:

ProA affinity purified

Molecular weight:

54 kDa

Applications:

IHC

Usage:

IHC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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