Background:

The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB. RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of a ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The C-terminus of RPA 32 can specfically intereact with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, RPA 32 binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA 32 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3.

Specificity:

RPA32/RPA2 (phospho T21) polyclonal antibody detects endogenous levels of RPA32/RPA2 protein only when phosphorylated at T21.

Alternative names:

RP-A p32, RF-A protein 2, RP-A p34, RPA2, REPA2, RPA32, RPA34, 60S acidic ribosomal protein P1, AA409079, AI325195, AU020965, ik:tdsubc_2g1, M(2)21C, MGC137236, OTTHUMP00000004008, p32, p34, RCJMB04_6d17 replication protein A2, 32kDa, REPA2, Replication factor A protein 2, Replication protein A 32 kDa subunit, Replication protein A 32kDa subunit, Replication protein A 34 kDa subunit, Replication protein A, Replication Protein A2 (32kDa), Replication protein A2, Replication protein A2, 32kDa, RF-A protein 2, Rf-A2, RFA, RFA2, RP-A p32, RP-A p34, RP21C, RPA 2, RPA 32, RPA, Rpa2, RPA32, RPA34, RpLP1, RpP2, xx:tdsubc_2g1, zgc:109822,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic phospho-peptide corresponding to residues surrounding Thr21 of human RPA32/RPA2.

Purification & Purity:

ProA affinity purified

Molecular weight:

32 kDa

Applications:

WB IP

Usage:

WB:1:1,000

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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