Background:

Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.

Specificity:

USP28 polyclonal antibody detects endogenous levels of USP28 protein.

Alternative names:

USP28, KIAA1515, Deubiquitinating enzyme 28, KIAA1515, Ubiquitin carboxyl terminal hydrolase 28, Ubiquitin carboxyl-terminal hydrolase 28, ubiquitin carboxyl-terminal hydrolase 28 variant 1, Ubiquitin specific peptidase 28, Ubiquitin specific processing protease 28, Ubiquitin specific protease 28, Ubiquitin thioesterase 28, Ubiquitin thiolesterase 28, Ubiquitin-specific-processing protease 28, UBP28, USP 28, USP28, USP28 protein,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within human USP28 aa 550-700.

Purification & Purity:

Protein affinity purified.

Molecular weight:

122 kDa

Applications:

WB FC

Usage:

WB:1:200-1:1,000 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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