Background:

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP14 (ubiquitin specific peptidase 14), also known as TGT (tRNA-guanine transglycosylase), is a cytoplasmic protein that belongs to the ubiquitin-specific processing family of deubiquitinating enzymes. Existing as a homodimer within the cell, USP14 functions to cleave ubiquitin residues from both ubiquitinylated proteins and ubiquitin-fused precursors, thereby saving these proteins from proteasomal degradation. In mice, defects or mutations in the gene encoding USP14 cause retarded growth or fetal death, indicating that USP14 plays a key role in early developmental processes. Multiple isoforms of USP14 are expressed due to alternative splicing events.

Specificity:

Usp14 polyclonal antibody detects endogenous levels of Usp14 protein.

Alternative names:

USP14, TGT, Deubiquitinating enzyme 14, TGT, tRNA guanine transglycosylase 60 kD subunit, tRNA guanine transglycosylase, Ubiquitin carboxyl terminal hydrolase 14, Ubiquitin carboxyl-terminal hydrolase 14, Ubiquitin specific peptidase 14, Ubiquitin specific processing protease 14, Ubiquitin specific protease 14, Ubiquitin thiolesterase 14, Ubiquitin-specific-processing protease 14, UBP14, USP 14, USP14,

Species reactivity:

Human, Zebrafish

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

56 kDa

Applications:

WB ICC IF IHC FC

Usage:

WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com