Background:

Ube2N, a member of the E2 Ubiquitin-conjugating enzyme family, completes the second step of ubiquitination reactions, essentially targeting proteins for proteasomic degradation. When proteins are modified with ubiquitin in an important cellular mechanism targeting abnormal or short lived proteins, ubiquitin, a short protein of 76 amino acids, attaches to a lysine residue resting on the target protein. Multiple cycles of ubiquitination create a polyubiquitin chain that the proteasome recognizes and subsequently triggers the ATP-dependent unfolding of the target protein. This allows protoeolytic degradation of the target protein. These degradation products are highly expressed in heart and skeletal muscles. Ube2N moderates the transcriptional activation of target genes, affects the progress of cell differentiation and aging, and also influences the DNA repair pathway, further adding to the survival of cells after DNA damage.

Specificity:

Ube2N polyclonal antibody detects endogenous levels of Ube2N protein.

Alternative names:

UBE2N, BLU, Bendless like ubiquitin conjugating enzyme, Bendless-like ubiquitin-conjugating enzyme, BLU, EC 6.3.2.19, Epididymis secretory protein Li 71, HEL-S-71, Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds, MGC131857, MGC8489, UBC 13, Ubc13, UbCH ben, UbcH-ben, UbcH13, UBCHBEN, Ube 2N, Ube2n, UBE2N, Ubiquitin carrier protein N, Ubiquitin conjugating enzyme E2 N, Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13), Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast), Ubiquitin conjugating enzyme E2N, Ubiquitin protein ligase N, Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N, Yeast UBC13 homolog,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within human Ube2N aa 1-150.

Purification & Purity:

ProA affinity purified

Molecular weight:

17 kDa

Applications:

WB

Usage:

WB:1:500-1:2,000

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com