Background:

The ubiquitin activating enzyme E1 (UBE1) catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. Specifically, UBE1 functions to adenylate the C-terminal glycine residue of ubiquitin, a reaction that is ATP-dependent and is proceeded by the formation of a thiolester bond with a cysteine residue of UBE1. The UBE1-activated ubiquitin is then transferred to a ubiquitin conjugated enzyme, which donates the ubiquitin residue to target substrates. The UBE1 gene is an example of an X-Y homologous gene, which is X-linked with a distinct Y-linked gene in many mammals. However, no UBE1 homolog is detectable on the human Y chromosome. UBE1 is thought to escape X inactivation in humans.

Specificity:

UBA1 polyclonal antibody detects endogenous levels of UBA1 protein.

Alternative names:

UBA1, A1S9, A1S9 protein, A1S9T and BN75 temperature sensitivity complementing, A1S9T, A1ST, AMCX1, CFAP124, CTD-2522E6.1, GXP 1, GXP1, MGC4781, POC20, POC20 centriolar protein homolog, Protein A1S9, SMAX2, Uba1, UBA1, ubiquitin-activating enzyme E1 homolog A, UBA1, UBA1A, UBE 1, UBE 1X, UBE1, UBE1X, Ubiquitin activating enzyme E1, Ubiquitin-activating enzyme E1, Ubiquitin-like modifier-activating enzyme 1,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

118 kDa

Applications:

WB ICC/IF IHC FC

Usage:

WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:100-1:500 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com