Background:

SNRPA (small nuclear ribonucleoprotein polypeptide A), also known as U1A (U1 snRNP protein A), is a component of the RNA spliceosome, a complex of proteins that are required for the precise excision of introns from pre-messenger RNA (pre-mRNA). Localizing to the nucleus, SNRPA contains two RRM (RNA recognition motif) domains, namely RRM1 and RRM2, and RRM1 specifically associates with the stem loop II of U1 snRNA (small nuclear RNA). In addition to functioning as a component of the U1 snRNP, SNRPA negatively regulates polyadenylation of SNRPA pre-mRNA, thereby negatively regulating itself. By inhibiting the addition of a polyA tail that would allow the pre-mRNA to mature, SNRPA causes the nuclear exosome degradation of the SNRPA pre-mRNA. At least 16% of cellular SNRPA also functions in an snRNP-free form (SF-A) that complexes with a group of non-snRNP proteins.

Specificity:

U1A polyclonal antibody detects endogenous levels of U1A protein.

Alternative names:

U1 snRNP A, U1-A, U1A, SNRPA, fc19d01, Mud1, small nuclear ribonucleoprotein polypeptide A, snRNP A, snRNP protein A, SNRPA, SNRPA, U1 small nuclear ribonucleoprotein A, U1 small nuclear RNP specific A, U1 snRNP A, U1 snRNP specific protein A, U1-A, U1A, wu:fc19d01, zgc:77810,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein corresponding to N-terminal human U1A.

Purification & Purity:

ProA affinity purified

Molecular weight:

31 kDa

Applications:

WB ICC IF IHC IP

Usage:

WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 IP:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com