Background:

The transformation of a normal cell to one that is malignant can result from mutations in genes that encode proteins with key regulatory functions. Examples include the retinoblastoma gene product (Rb p110), p53, VHL and APC. Using a novel cloning strategy that allows the isolation of previously uncharacterized genes encoding selectable recessive phenotypes, an additional tumor suppressor gene has been identified. This gene, termed tsg 101 for tumor susceptibility gene 101, encodes a stathmin binding domain protein. When expression of this growth inhibitory gene is blocked in NIH/3T3 cells using antisense mRNA, the cells exhibit a transformed phenotype and are tumorigenic in SL6 mice.

Specificity:

TSG101 polyclonal antibody detects endogenous levels of TSG101 protein.

Alternative names:

TSG101, ESCRT I complex subunit TSG101, ESCRT-I complex subunit TSG101, TS101, TSG 10, TSG 101, TSG10, Tsg101, Tumor susceptibility gene 10, Tumor susceptibility gene 101, Tumor susceptibility gene 101 protein, Tumor susceptibility protein, Tumor susceptibility protein isoform 3, VPS 23, VPS23,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

44 kDa

Applications:

WB ICC/IF IHC FC

Usage:

WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com