Background:

Tyrosinase (TYR), a type I membrane protein and copper-containing enzyme, is involved in the production of melanin, the primary pigment found in vertebrates. Melanin biogenesis requires the enzymatic activity of TYR, which catalyzes the critical and rate-limiting step of tyrosine hydroxylation in the biosynthesis of melanin. Defects effecting TYR activity result in various forms of albinism. The TYR-related proteins, TRP1 and TRP2, are also specifically expressed in melanocytes, and they likewise contribute to the synthesis of melanin within the melanosomes. The TRPs, including TYR, all share a similar transmembrane region, contain two metal-binding regions and a cysteine-rich epidermal growth factor motif, and are localized in the melanosomal membrane. These proteins, however, have distinct catalytic activity, and they individually contribute to the biosynthesis of melanin biopolymers. The TRPs are believed to exists as a multi-enzyme complex, as these proteins form aggregates together, and the expression of TRP1 also helps stabilize TYR in melanocytes.

Specificity:

TRP1 polyclonal antibody detects endogenous levels of TRP1 protein.

Alternative names:

DHICA oxidase, TRP, TRP-1, TRP1, TYRP1, CAS2, TYRP, TYRRP, 5, 5, 6 dihydroxyindole 2 carboxylic acid oxidase, 6-dihydroxyindole-2-carboxylic acid oxidase, b-PROTEIN, CAS2, Catalase B, CATB, DHICA oxidase, Glycoprotein 75, GP75, Melanoma antigen gp75, OCA3, TRP, TRP-1, TRP1, Tyrosinase related protein 1, Tyrosinase-related protein 1, TYRP, TYRP1, TYRP1, TYRRP,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

72 kDa

Applications:

WB ICC IHC IP

Usage:

WB:1:500-1:2,000 ICC:1:50-1:100 IHC:1:50-1:200 IP:1:10-1:50

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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