Background:

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system. Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas. TrkB is overexpressed in tumors such as neuroblastoma, prostate adenocarcinoma and pancreatic ductal adenocarcinoma. In neuroblastomas overexpression of TrkB correlates with unfavorable disease outcome when autocrine loops signaling tumor survival are potentiated by additional overexpression of brain-derived neurotrophic factor (BDNF). An alternatively spliced truncated TrkB isoform lacking the kinase domain is overexpressed in Wilms’ tumors and this isoform may act as a dominant-negative to TrkB signaling. Altered TrkC expression and corresponding gene mutations are seen in various forms of cancer, with increased expression a positive prognostic indicator in patients with medulloblastoma.

Specificity:

TrkA/B/C polyclonal antibody detects endogenous levels of TrkA/B/C protein.

Alternative names:

Trk-A, NTRK1, Trk-B, NTRK2, Trk-C, NTRK3, BDNF/NT-3 growth factors receptor, gp140trk, GP145-TrkB, GP145-TrkC, High affinity nerve growth factor receptor, MTC, Neurotrophic tyrosine kinase receptor type 1, Neurotrophic tyrosine kinase receptor type 2, Neurotrophic tyrosine kinase receptor type 3, NT-3 growth factor receptor, NTRK1, NTRK2, NTRK3, p140-TrkA, TRK, Trk-A, Trk-B, Trk-C, TRK1-transforming tyrosine kinase protein, TRKA, TRKB, TrkB tyrosine kinase, TRKC, TrkC tyrosine kinase, Tropomyosin-related kinase A, Tropomyosin-related kinase B, Tyrosine kinase receptor A, Tyrosine kinase receptor,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

140 kDa

Applications:

WB ICC IHC

Usage:

WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com