Background:

eRF3a (eukaryotic peptide chain release factor subunit 3a), also known as GSPT1 (G1 to S phase transition 1), is a 499 amino acid protein that belongs to the GTP-binding elongation factor family and is involved in the regulation of cell growth, specifically via control of translation termination. Human eRF3a shares 94% sequence identity with its mouse counterpart, suggesting a conserved function between species. The gene encoding eRF3a maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth.

Specificity:

eRF3a (D128) polyclonal antibody detects endogenous levels of eRF3a protein.

Alternative names:

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor subunit 3a; eRF3a; G1 to S phase transition protein 1 homolog; GSPT1; ERF3A

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 100-150 of Human eRF3a.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 80 kDa

Applications:

WB IHC

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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