Background:

Terminally differentiating mammalian epidermal cells acquire an insoluble, 10 to 20 nm thick protein deposit on the intracellular surface of the plasma membrane known as the cross-linked cell envelope (CE). The CE is a component of the epidermis that is generated through formation of disulfide bonds and g-glutamyl-lysine isodipeptide bonds, which are formed by the action of transglutaminases (TGases). TGases are intercellularly localizing, Ca2+-dependent enzymes that catalyze the formation of isopeptide bonds by transferring an amine on to glutaminyl residues, thereby cross-linking glutamine residues and lysine residues in substrate proteins. TGases influence numerous biological processes, including blood coagulation, epidermal differentiation, seminal fluid coagulation, fertilization, cell differentiation and apoptosis. Human keratinocyte transglutaminase (TGase1) is a membrane associated, 817 amino acid protein. Human tissue transglutaminase (TGase2) is an endothelial cell specific, 687 amino acid protein.

Specificity:

TGase2/TGM2 polyclonal antibody detects endogenous levels of TGase2/TGM2 protein.

Alternative names:

TG(C), TGC, TGase C, TGase H, TGase-2, TGM2, ALPHA SUBUNIT, C polypeptide, EC 2.3.2.13 antibodyepididymis secretory protein Li 45, G alpha h, G[a]h, Gh CLASS G ALPHA h, GNAH, GNAH G PROTEIN, H POLYPEPTIDE, HEL-S-45, Protein glutamine gamma glutamyltransferase 2, Protein-glutamine gamma-glutamyltransferase 2, TG 2, TG(C), TG2, TGase C, TGase H, TGase-2, TgaseII, TGC, TGC GUANINE NUCLEOTIDE BINDING PROTEIN, TGM2, TGM2, Tissue transglutaminase, Transglutaminase 2, Transglutaminase 2 C polypeptide, Transglutaminase C, Transglutaminase H, Transglutaminase-2, tTG, tTGas,

Species reactivity:

Human, Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

77 kDa

Applications:

WB IHC IP

Usage:

WB:1:500-1:2,000 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com