Background:

T helper (Th) lymphocytes differentiate into two unique subsets, Th1 and Th2, which differ both in function and in the cytokines they secrete. Th1 and Th2 cytokines promote the growth and differentiation of their subset, and inhibit the growth and differentiation of the opposing subset. T-bet (T box expressed in T cells) is a Th1-specific T box transcription factor that controls the expres-sion of the Th1 cytokine, IFN-γ. T-bet also converts effector Th2 cells into the opposing Th1 subset. T-bet is selectively expressed in Th1 cells. The level of T-bet expression is increased by signals mediated by the T cell receptor (TCR). IL-12 also induces an increase in the level of T-bet. T-bet was originally isolated from nuclear extracts of resting and PMA/ionomycin-activated AE7 cells. T-bet is expressed in low levels in AE7 cells, and in increased levels in stimulated AE7.

Specificity:

Tbx21 polyclonal antibody detects endogenous levels of Tbx21 protein.

Alternative names:

T-box protein 21, Tbx21, Tbet, Tblym, T bet, T box 21, T box expressed in T cells, T box protein 21, T box transcription factor TBX21, T cell specific T box transcription factor, T cell specific T box transcription factor T bet, T PET, T-box protein 21, T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet, TBET, TBLYM, TBX 21, Tbx21, TBX21_HUMAN antibody TPET, Transcription factor TBLYM,

Species reactivity:

Human, Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide within human T-bet aa 480-530.

Purification & Purity:

Peptide affinity purified.

Molecular weight:

58 kDa

Applications:

WB FC

Usage:

WB:1:500 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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