Background:

The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2 and SUMO-3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include IkBa, MDM2, p53, PML and Ran GAP1. SUMO-2 and SUMO-3 contribute to a greater percentage of protein modification than does SUMO-1, and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates b-Amyloid generation and may be critical in the onset or progression of Alzheimer's disease.

Specificity:

Sumo 2/3 polyclonal antibody detects endogenous levels of Sumo 2/3 protein.

Alternative names:

SUMO-2, Smt3B, SUMO2, SUMO-3, Smt3A, SUMO3, HSMT3, MGC117191, OTTHUMP00000115275, OTTHUMP00000115276, OTTHUMP00000115277, Sentrin 2, Small ubiquitin like modifier 2, Small ubiquitin related modifier 2, small ubiquitin-like modifier 3, small ubiquitin-related modifier 3, SMT3 homolog 1, SMT3 homolog 2, SMT3 suppressor of mif two 3 homolog 1, SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae), SMT3 suppressor of mif two 3 homolog 2, SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae), SMT3 suppressor of mif two 3 homolog 3, SMT3A, SMT3B, SMT3H1, SMT3H2, Sumo2, Sumo3, Ubiquitin like protein SMT3A, Ubiquitin like protein SMT3B,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

12 kDa

Applications:

WB ICC/IF IHC

Usage:

WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com