Background:

The silent information regulator (SIR2) family of genes are highly-conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues. SIRT1 is highly expressed in heart, brain and skeletal muscle, with low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lys 382.

Specificity:

SIRT1 polyclonal antibody detects endogenous levels of SIRT1 protein.

Alternative names:

hSIRT1, hSIR2, SIRT1, 75SirT1, hSIR2, hSIRT1, HST2, S. cerevisiae, homolog of, NAD dependent deacetylase sirtuin 1, NAD dependent protein deacetylase sirtuin 1, OTTHUMP00000198111, OTTHUMP00000198112, Regulatory protein SIR2 homolog 1, SIR1, SIR2, SIR2 like 1, SIR2 like protein 1, SIR2, S.cerevisiae, homolog-like 1, SIR2-like protein 1, SIR2ALPHA, SIR2L1, Sirt1, SirtT1 75 kDa fragment, Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), Sirtuin 1, Sirtuin type 1,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

110 kDa

Applications:

WB ICC/IF IHC IP

Usage:

WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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