Background:

Cumulative damage to lung tissue by Neutrophil Elastase is responsible for the development of pulmonary emphysema, an irreversible lung disease characterized by loss of lung elasticity. a 1-antitrypsin (AAT), a 394 amino acid hepatic acute phase protein, predominantly inhibits Neutrophil Elastase. AAT is highly expressed in liver and in cultured hepatoma cells and, to a lesser extent, in macrophages. AAT is a highly polymorphic glycosylated serum protein with characteristic isoelectric-focusing patterns for most variants. The gene encoding AAT maps to a region of human chromosome 14 that includes a related serine protease inhibitor (serpin) gene which encodes corticosteroid-binding globulin. Oxidation of the methionine 358 residue in the active center of AAT results in a dramatic decrease in inhibitory activity towards elastase. AAT also has a moderate affinity for plasmin and Thrombin. AAT deficiency is associated with a 20-30 fold increased risk of precocious pulmonary emphysema.

Specificity:

SERPINA1 polyclonal antibody detects endogenous levels of SERPINA1 protein.

Alternative names:

SERPINA1, A1A, A1AT, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 antitrypsin null, Alpha 1 protease inhibitor, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, alpha1 proteinase inhibitor, Alpha1AT, Dom1, PI, PI1, PRO2275, Serine (or cysteine) proteinase inhibitor clade A member 1, Serine protease inhibitor 1-1, Serine protease inhibitor A1a, Serpin A1, Serpin A1a, Serpin peptidase inhibitor clade A member 1, Serpina1, Short peptide from AAT, SPAAT, Spi1-1,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

47 kDa

Applications:

WB ICC/IF IHC IP

Usage:

WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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