Background:

The mammalian Runt-related transcription factor (RUNX) family comprises three members, RUNX1 (also designated AML-1, PEBP2αB, CBFA2), RUNX2 (also designated AML-3, PEBP2αA, CBFA1, Osf2) and RUNX3 (also designated AML-2, PEBPαC, CBFA3). RUNX family members are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX2 is essential for skeletal mineralization in that it stimulates osteoblast differentiation of mesenchymal stem cells, promotes chondrocyte hypertrophy and contributes to endothelial cell migration and vascular invasion of developing bones. Regulating RUNX2 expression may be a useful therapeutic tool for promoting bone formation. Mutations in the C-terminus of RUNX2 are associated with cleidocranial dysplasia syndrome, an autosomal-dominant skeletal dysplasia syndrome that is characterized by widely patent calvarial sutures, clavicular hypoplasia, supernumerary teeth, and short stature.

Specificity:

RUNX2 polyclonal antibody detects endogenous levels of RUNX2 protein.

Alternative names:

CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A, RUNX2, AML3, CBFA1, OSF2, PEBP2A, Acute myeloid leukemia 3 protein, Alpha subunit 1, AML3, CBF alpha 1, CBF-alpha-1, CBFA1, CCD, CCD1, Cleidocranial dysplasia 1, Core binding factor, Core binding factor runt domain alpha subunit 1, Core binding factor subunit alpha 1, Core-binding factor subunit alpha-1, MGC120022, MGC120023, Oncogene AML 3, Oncogene AML-3, OSF 2, OSF-2, OSF2, Osteoblast specific transcription factor 2, Osteoblast-specific transcription factor 2, OTTHUMP00000016533, PEA2 alpha A, PEA2-alpha A, PEA2aA, PEBP2 alpha A, PEBP2-alpha A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, Polyomavirus enhancer binding protein 2 alpha A subunit, Polyomavirus enhancer-binding protein 2 alpha A subunit, Runt domain, Runt related transcription factor 2, Runt-related transcription factor 2, RUNX2, RUNX2, SL3 3 enhancer factor 1 alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core binding factor alpha A subunit, SL3/AKV core-binding factor alpha A subunit,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide of C-terminal human RUNX2.

Purification & Purity:

Peptide affinity purified.

Molecular weight:

56.6 kDa

Applications:

WB IHC ICC

Usage:

WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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