Background:

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Specificity:

RPA32/RPA2 polyclonal antibody detects endogenous levels of RPA32/RPA2 protein.

Alternative names:

RP-A p32, RF-A protein 2, RP-A p34, RPA2, REPA2, RPA32, RPA34, 60S acidic ribosomal protein P1, AA409079, AI325195, AU020965, ik:tdsubc_2g1, M(2)21C, MGC137236, OTTHUMP00000004008, p32, p34, RCJMB04_6d17 replication protein A2, 32kDa, REPA2, Replication factor A protein 2, Replication protein A 32 kDa subunit, Replication protein A 32kDa subunit, Replication protein A 34 kDa subunit, Replication protein A, Replication Protein A2 (32kDa), Replication protein A2, Replication protein A2, 32kDa, RF-A protein 2, Rf-A2, RFA, RFA2, RP-A p32, RP-A p34, RP21C, RPA 2, RPA 32, RPA, Rpa2, RPA32, RPA34, RpLP1, RpP2, xx:tdsubc_2g1, zgc:109822 antibody

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within C-terminal human RPA32/RPA2.

Purification & Purity:

Protein A purified.

Molecular weight:

32 kDa

Applications:

WB IP IHC

Usage:

WB:1:500-1:2,000 IP:1:10-1:50 IHC:1:50-1:200

Storage:

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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