Background:

In the intact cell, DNA is closely associated with histones and other nuclear proteins to form chromatin.The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation, and an increased accessiblity of DNA to transcription factors. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. The retinoblastoma binding proteins RbAp46 and RbAp48 have been identified as histone binding proteins, and they are components of the histone deacetylase complex.

Specificity:

RbAP48 polyclonal antibody detects endogenous levels of RbAP48 protein.

Alternative names:

CAF-1 subunit C, CAF-I 48 kDa subunit, CAF-I p48, RBBP-4, RBBP4, RBAP48, CAF I p48, CAF-1 subunit C, CAF-I 48 kDa subunit, CAF-I p48, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Chromatin assembly factor/CAF 1 p48 subunit, Histone-binding protein RBBP4, MSI1 protein homolog, Nucleosome-remodeling factor subunit RBAP48, NURF55, RB binding protein 4 chromatin remodeling factor, RbAp 48, RBAP48, RBBP-4, RBBP4, RBBP4, Retinoblastoma binding protein 4, Retinoblastoma binding protein p48, Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

48 kDa

Applications:

WB ICC IHC FC IP

Usage:

WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com