Background:

Ras superfamily is a protein superfamily of small GTPases, which are all related, to a degree, to the Ras protein subfamily (the key human members of which are KRAS, NRAS, and HRAS). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. This antibody is predicted to react with H-Ras, N-Ras and K-Ras.

Specificity:

Ras polyclonal antibody detects endogenous levels of Ras protein.

Alternative names:

Ras, C-BAS/HAS, c-H-ras, C-HA-RAS1, CTLO, GTPase HRas, GTPase KRas, GTPase NRas, H-Ras-1, H-RASIDX, Ha-Ras, HAMSV, HRAS, HRAS1, K RAS2A, K RAS2B, K RAS4A, K RAS4B, K-RAS, KRAS, KRAS1, KRAS2, N-RAS, N-terminally processed, NRAS, NRAS1, p21ras, RASH, RASH1, RASK2, Transforming protein p21, v Ha ras Harvey rat sarcoma viral oncogene homolog, v Ki ras2 Kirsten rat sarcoma viral oncogene homolog, v ras neuroblastoma RAS viral oncogene homolog,

Species reactivity:

Human, mouse, rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

peptide

Purification & Purity:

Peptide affinity purified

Molecular weight:

21 kDa

Applications:

WB ICC IHC FC

Usage:

WB:1:1,000 ICC:1:200 IHC:1:200 FC:1:100-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com