Background:

The small Ras related protein Ran, also called TC4, is a nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The abundant GEF RCC1 (regulator of chromosome condensation 1) increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A protein designated Ran BP1 has no intrinsic GAP activity and functions as a GEF inhibitor deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. Ran BP2 has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex.

Specificity:

RanGAP1 polyclonal antibody detects endogenous levels of RanGAP1 protein.

Alternative names:

RanGAP1, RANGAP1, KIAA1835, SD, Fug 1, Fug1, GTPase-activating protein, RAN, 1, KIAA1835, MGC20266, OTTHUMP00000028918, OTTHUMP00000198755, OTTHUMP00000198756, OTTHUMP00000198757, OTTHUMP00000198758, RAGP1, Ran 1, RAN GTPase activating protein 1, Ran GTPase-activating protein 1, Ran1, RANGAP 1, RANGAP, RanGAP1, SD, Segregation distorter homolog, Segregation distortion,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Purification & Purity:

ProA affinity purified

Molecular weight:

64 kDa

Applications:

WB ICC IF IHC FC IP

Usage:

WB:1:500-1:2,000 ICC/IF:1:100 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com