Background:

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.

Specificity:

Rad21 polyclonal antibody detects endogenous levels of Rad21 protein.

Alternative names:

hHR21, NXP-1, RAD21, HR21, KIAA0078, NXP1, SCC1, CDLS4, Double-strand-break repair protein rad21 homolog, hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear matrix protein 1, NXP-1, NXP1, Protein involved in DNA double-strand break repair, RAD21, RAD21 homolog (S. pombe), RAD21 homolog, RAD21, Scc1, SCC1 homolog antibody

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within human Rad21 aa 250-440.

Purification & Purity:

Protein affinity purified.

Molecular weight:

120 kDa, predicted band size 72 kDa

Applications:

WB ICC IHC

Usage:

WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:100-1:400

Storage:

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com