Background:

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. Receptor for activated C kinases, termed RACKs, are intracellular receptors for activated PKC that may be involved in the activation-induced translocation of PKC. RACK1 (receptor for activated C kinase 1) is a 317 amino acid G protein b subunit-like protein that functions as a RACK and inhibits the activity of Src tyrosine kinases. In response to PKC activation, the intracellular localization of RACK1 and PKC bII changes, and RACK1 and PKC bII co-localize to the same sites. RACK1 is therefore thought to be a shuttling protein for PKC bII. A deficit in RACK1 may be associated with impaired PKC activation in the aging brain. The RACK1 gene is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.pombe, S.cerevisiae, K.lactis, E.gossypii, M.grisea, N.crassa, A.thaliana, rice and P.falciparum, and maps to human chromosome 5q35.3.

Specificity:

RACK1 polyclonal antibody detects endogenous levels of RACK1 protein.

Alternative names:

HLC-7, RACK1, GNB2L1, Cell proliferation-inducing gene 21 protein, GBLP, Gnb2-rs1, Gnb2l1, Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1, Guanine nucleotide binding protein beta polypeptide 2 like 1, Guanine nucleotide binding protein beta subunit 2 like 1, Guanine nucleotide binding protein beta subunit like protein 12.3, Guanine nucleotide binding protein subunit beta 2 like 1, Guanine nucleotide binding protein subunit beta like protein 12.3, Guanine nucleotide-binding protein subunit beta-2-like 1, Guanine nucleotide-binding protein subunit beta-like protein 12.3, H12.3, HLC-7, Human lung cancer oncogene 7 protein, lung cancer oncogene 7, OTTHUMP00000223704, OTTHUMP00000223870, OTTHUMP00000223891, OTTHUMP00000223893, OTTHUMP00000223900, OTTHUMP00000223902, OTTHUMP00000223930, OTTHUMP00000223931, PIG21, Proliferation inducing gene 21, Protein homologous to chicken B complex protein guanine nucleotide binding, RACK1, Receptor for activated C kinase 1, Receptor for activated C kinase, Receptor of activated protein kinase C 1,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein corresponding to C-terminal human RACK1.

Purification & Purity:

ProA affinity purified

Molecular weight:

35 kDa

Applications:

WB IHC FC

Usage:

WB:1:1,000-1:2,000 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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