Background:

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs.

Specificity:

Pumilio 1 polyclonal antibody detects endogenous levels of Pumilio 1 protein.

Alternative names:

HsPUM, Pumilio-1, PUM1, KIAA0099, PUMH1, HsPUM, KIAA0099, PUM 1, PUM L1, Pum1, PUM1, PUMH 1, PUMH, PUMH1, pumilio homolog 1 (Drosophila), Pumilio homolog 1, Pumilio-1, Pumilio1, PUML 1, PUML1,

Species reactivity:

Human, Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein within human Pumilio 1 aa 1-200.

Purification & Purity:

ProA affinity purified

Molecular weight:

126 kDa

Applications:

WB ICC IF IHC FC IP

Usage:

WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50

Storage:

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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