Background:

A class of proteins termed type 1 protein arginine N-methyltransferase (PRMT) enzymes contribute to posttranslational modification of RNA-binding proteins, but differ in substrate specificities, oligomerization properties and subcellular localization. PRMT1, the predominant form in mammalian cells, is located in the nucleus, while PRMT3 is present in the cytoplasm. At the carboxy-terminus, interleukin enhancer-binding factor 3 (ILF3) binds PRMT1, thereby regulating PRMT1 activitiy. Alternative mRNA splicing of the PRMT gene results in three isoforms of PRMT1 that differ in their amino-terminus regions. All three splice variants of PRMT1 are enzymatically active. PRMT3 recognizes and binds to RNA-associated substrates with a zinc-finger domain in its amino-terminus. The zinc-liganded form of this domain is required for the enzyme to recognize RNA-associated substrates.

Specificity:

PRMT1 polyclonal antibody detects endogenous levels of PRMT1 protein.

Alternative names:

PRMT1, ANM 1, ANM1, ANM1, HCP 1, HCP1, Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2, Heterogeneous nuclear ribonucleoproteins methyltransferase like 2, Heterogeneous nuclear ribonucleoproteins methyltransferase like2, Histone-arginine N-methyltransferase PRMT1, HMT 2, HMT1 (hnRNP methyltransferase S. cerevisiae) like 2, HMT1 hnRNP methyltransferase, HMT1 hnRNP methyltransferase like 2 (S. cerevisiae), HMT1 hnRNP methyltransferase like 2, HMT2, HRMT1 L2, HRMT1L 2, HRMT1L2, Human mRNA for suppressor for yeast mutant, Human mRNA for suppressor for yeast mutant complete cds, Interferon receptor 1 bound protein 4, Interferon receptor 1 bound protein4, Interferon receptor 1-bound protein 4, Interferon receptor 1bound protein 4, IR1 B4, IR1B 4, IR1B4, Mrmt 1, Mrmt1, PRMT 1, PRMT1, Protein arginine methyltransferase 1, Protein arginine N methyltransferase 1, Protein arginine N methyltransferase1, Protein arginine N-methyltransferase 1, R1B4, S. cerevisiae like 2,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

42 kDa

Applications:

WB ICC/IF IHC CHIP

Usage:

WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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