Background:

Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLCε. PLC γ1 is widely distributed in bronchiolar epithelium, type I and II pneumocytes and fibroblasts of the interstitial tissue. Actin-regulatory protein Villin is tyrosine phosphorylated and associates with PLC γ1 in the brush border of intestinal epithelial cells. Villin regulates PLC γ1 activity by modifying its own ability to bind phosphatidylinositol 4,5-biphosphate. PLC γ1 binds Integrin α1/β1 and modulates Integrin α1/β-specific adhesion. PLC γ1 and Ca2+ play a direct role in VEGF-regulated endothelial growth, however this signaling pathway is not linked to FGF-mediated effects in primary endothelial cells. PLC γ1 is rapidly activated in response to growth factor stimulation and plays an important role in regulating cell proliferation and differentiation. It may also have a protective function during cellular response to oxidative stress.

Specificity:

PLCG1 polyclonal antibody detects endogenous levels of PLCG1 protein.

Alternative names:

PLC-II, PLC-gamma-1, PLCG1, 1 phosphatidyl D myo inositol 4 5 bisphosphate, 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1, 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1, Inositoltrisphosphohydrolase, Monophosphatidylinositol phosphodiesterase, NCKAP3, Phosphatidylinositol phospholipase C, Phosphoinositidase C, Phosphoinositide phospholipase C, Phosphoinositide phospholipase C-gamma-1, Phospholipase C 148, Phospholipase C gamma 1, Phospholipase C-gamma-1, Phospholipase C-II, PLC gamma 1, PLC II, PLC-148, PLC-gamma-1, PLC-II, PLC1, PLC148, Plcg1, PLCG1, PLCgamma1,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

149 kDa

Applications:

WB ICC IP

Usage:

WB:1:1,000 ICC:1:50-1:200

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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