Background:

An interferon-inducible, RNA-dependent protein serine/threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the a subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2a on serine-51 results in inhibition of translation. Molecular cDNA clones have been isolated from both human and mouse cells. The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. Three kinds of regulation of PKR enzymatic activity have been described. These include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation and post-translational regulation by RNA mediated autophosphorylation.

Specificity:

PKR polyclonal antibody detects endogenous levels of PKR protein.

Alternative names:

eIF-2A protein kinase 2, PKR, Protein kinase R, EIF2AK2, Double stranded RNA activated protein kinase;, E2AK2, eIF-2A protein kinase 2, EIF2AK1, EIF2AK2, Eukaryotic translation initiation factor 2 alpha kinase 2, Eukaryotic translation initiation factor 2-alpha kinase 2, HGNC:9437, Interferon induced double stranded RNA activated protein kinase, Interferon inducible elF2 alpha kinase, Interferon inducible RNA dependent protein kinase, Interferon-induced, double-stranded RNA-activated protein kinase, Interferon-inducible RNA-dependent protein kinase, MGC126524, P1/eIF-2A protein kinase, P1/eIF2A protein kinase, p68 kinase, PKR, PPP1R83, PRKR, Protein kinase interferon inducible double stranded RNA dependent, Protein kinase RNA activated, Protein kinase RNA-activated, Protein phosphatase 1 regulatory subunit 83, Serine/threonine protein kinase TIK, Tyrosine protein kinase EIF2AK2,

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

68 kDa

Applications:

WB ICC/IF IHC IP

Usage:

WB:1:1,000-1:5,000 ICC:1:50-1:100 IHC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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