Background:

Normal adjustment to changes in blood glucose levels depends on Insulin signaling as well as enzymes involved in the regulation of gluconeogenesis. Pathological changes to this process are central to the type 2 diabetes phenotype. Phosphoenolpyruvate carboxykinase (PEPCK) plays an important role in this process by stimulating hepatic glucose production. PEPCK expression increases in response to glucagon and glucocorticoids, while Insulin suppresses expression. Modulation of the signals governing PEPCK levels present a potential therapeutic approach to the treatment of Insulin resistance and consequently obesity. The cytosolic form of PEPCK, known as PEPCK-C, and the mitochondrial form, known as PEPCK-M, are encoded by two different nuclear genes in mouse, human and chicken.

Specificity:

PCK2 polyclonal antibody detects endogenous levels of PCK2 protein.

Alternative names:

PEPCK-M, PCK2, PEPCK2, EC 4.1.1.32, GTP mitochondrial precursor, HGNC:8725, mitochondrial, Mitochondrial phosphoenolpyruvate carboxykinase 2, OTTHUMP00000164700, PCK2, PCKGM, PE, PEP carboxykinase, PEPCK, PEPCK deficiency mitochondrial, PEPCK M, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase [GTP], Phosphoenolpyruvate carboxykinase 2 (mitochondrial), Phosphoenolpyruvate carboxykinase 2 mitochondrial, Phosphoenolpyruvate carboxylase, Phosphopyruvate carboxylase,

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein

Purification & Purity:

ProA affinity purified

Molecular weight:

71 kDa

Applications:

WB ICC IF IHC FC

Usage:

WB:1:500-1:1,000 ICC:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100

Storage:

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com